| Identifier | HPI054456 |
| Interaction | NS5A <==> p53 |
| Role & State | --- |
| Interaction Type | IT:0000168,inhibit; IT:0000080,effect; IT:0000285,process; IT:0000114,activation; IT:0000227,bind; IT:0000174,repress; IT:0000068,transcription; |
| Biological Process | BP:0006915,apoptosis; BP:0009987,cellular process; |
| Biological Function | --- |
| Subcelluar Location | --- |
| Detection Method | DM:0000054,Flow cytometry; |
| Reference | 12101418_6, NS5A inhibits transcriptional transactivation by p53 in a dose-dependent manner by use of a reporter assay.
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| Reference | 12101418_8, The effect of NS5A on p53-mediated apoptosis was determined by flow cytometry in both NS5A permanently and transiently transfected Hep3B cells with exogenous p53.
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| Reference | 12379483_10, Taken together, these results suggest that NS5A may exert its influence on key cellular processes by functional associations with p53 and TBP.
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| Reference | 12379483_7, To determine the functional relevance of these associations, we found that NS5A inhibits the binding of both p53 and TBP to their DNA consensus binding sequences in vitro.
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| Reference | 12379483_9, Furthermore, NS5A repressed the p53 regulated p21 (WAF1) promoter and a synthetic promoter containing multiple p53 responsive DNA elements binding sites in HCT116 p53(+/+) cell line. p53-mediated transcriptional activation from both promoters was reduced approximately 3-5-fold following expression of NS5A.
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